Commonly used auxotrophic markers - Revision history

Suzia at 20:34, 11 July 2019

2019-07-11T20:34:17Z

Stacia at 20:28, 11 July 2019

2019-07-11T20:28:51Z

Show changes

Stacia at 19:08, 5 August 2014

2014-08-05T19:08:05Z

Stacia at 19:07, 5 August 2014

2014-08-05T19:07:19Z

Stacia at 19:05, 5 August 2014

2014-08-05T19:05:22Z

Stacia at 19:02, 5 August 2014

2014-08-05T19:02:52Z

Stacia at 19:00, 5 August 2014

2014-08-05T19:00:25Z

Maria at 19:46, 20 August 2013

2013-08-20T19:46:25Z

Gail at 19:42, 20 August 2013

2013-08-20T19:42:14Z

Maria at 21:30, 8 March 2012

2012-03-08T21:30:10Z

@@ Line 1: / Line 1: @@
-This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications.
+This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [https://www.yeastgenome.org/reference/S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications.
@@ Line 18: / Line 18: @@
 GGA-to-GAA missense change at codon 185
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001]
+[https://www.yeastgenome.org/reference/S000060944 Nakayashiki et al. 2001]
 |-
+|
@@ Line 62: / Line 62: @@
 G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000087056 Gai and Voytas, 2005]
+[https://www.yeastgenome.org/reference/S000087056 Gai and Voytas, 2005]
 |-
+|
@@ Line 75: / Line 75: @@
 frameshift (BglII site filled in at position 592)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000049198 Engebrecht and Roeder 1990]
+[https://www.yeastgenome.org/reference/S000049198 Engebrecht and Roeder 1990]
 |-
+|
@@ Line 97: / Line 97: @@
 no
+|
-Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000074003 Zhang et al. (2003)] for a discussion of this issue.
+Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See [https://www.yeastgenome.org/reference/S000074003 Zhang et al. (2003)] for a discussion of this issue.
+|
 kb deletion (-205 to 835)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042846 Struhl 1985]; [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000046453 Fasullo and Davis 1988]
+[https://www.yeastgenome.org/reference/S000042846 Struhl 1985]; [https://www.yeastgenome.org/reference/S000046453 Fasullo and Davis 1988]
 |-
+|
@@ Line 114: / Line 114: @@
 bp HindIII-HindIII internal deletion (305 to 492)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000040818 Scherer and Davis 1979]
+[https://www.yeastgenome.org/reference/S000040818 Scherer and Davis 1979]
 [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979]
 |-
@@ Line 156: / Line 156: @@
 EcoRI-ClaI internal deletion (163 to 649, 0.6 kb)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski and Hieter 1989]
+[https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989]
 |-
+|
@@ Line 174: / Line 174: @@
 GAC-to-AAC missense change at codon 300
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000043265 Hinnen et al. 1978];
+[https://www.yeastgenome.org/reference/S000043265 Hinnen et al. 1978];
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042541 Gaber and Culbertson 1982];
+[https://www.yeastgenome.org/reference/S000042541 Gaber and Culbertson 1982];
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000069760 Meira LB et al., 1995];
+[https://www.yeastgenome.org/reference/S000069760 Meira LB et al., 1995];
 [http://wiki.yeastgenome.org/index.php/CommunityW303.html Rodney Rothstein, Personal communication to SGD]
 [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979]
@@ Line 218: / Line 218: @@
 XhoI-HpaI internal deletion (1813 to 2864, 1.0 kb)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al. 1995]
+[https://www.yeastgenome.org/reference/S000047446 Winston et al. 1995]
 |-
+|
@@ Line 245: / Line 245: @@
 .45 kb deletion, EcoRI-EcoRI (-102 to 1352)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski and Hieter 1989]
+[https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989]
 |-
+|
@@ Line 258: / Line 258: @@
 .6 kb deletion, EcoRI-HindIII (-102 to 513)
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski and Hieter 1989]
+[https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989]
 |-
+|
@@ Line 271: / Line 271: @@
 GAG-to-TAG amber nonsense change at codon 83
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041995 McDonald et al. 1997]
+[https://www.yeastgenome.org/reference/S000041995 McDonald et al. 1997]
 |-
@@ Line 338: / Line 338: @@
 Ty1 insertion (transcribing left to right) at pos. 121
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000043686 Rose and Winston 1984]
+[https://www.yeastgenome.org/reference/S000043686 Rose and Winston 1984]
 |-
+|
@@ Line 384: / Line 384: @@
 |}
-The alleles listed below are described in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998].
+The alleles listed below are described in [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998].
@@ Line 402: / Line 402: @@
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000048958 (Aparicio et al. 1991)]
+[https://www.yeastgenome.org/reference/S000048958 (Aparicio et al. 1991)]
 |-
+|
@@ Line 415: / Line 415: @@
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
+[https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998]
 |-
@@ Line 429: / Line 429: @@
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
+[https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998]
 |-
+|
@@ Line 442: / Line 442: @@
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
+[https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998]
 |-
+|
@@ Line 455: / Line 455: @@
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
+[https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998]
 |-
@@ Line 465: / Line 465: @@
 <sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
-<sup>b</sup>All trp- strains are cold sensitive ([http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057172 Singh and Manney 1974]).
+<sup>b</sup>All trp- strains are cold sensitive ([https://www.yeastgenome.org/reference/S000057172 Singh and Manney 1974]).

@@ Line 312: / Line 312: @@
 Allows the selection of vectors carrying the TRP1 gene.
+|
-[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=395030 Bostein et al. 1979]
+[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=395030 Botstein et al. 1979]
 |-
+|

@@ Line 20: / Line 20: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade2 <em>ade2-1</em>]

@@ Line 96: / Line 96: @@
 no
+|
+chemically constructed deletion; reversion is undetectable (< 10(E-9) revertants in a culture). Causes histidine to be required for growth; allows selection of vectors carrying the HIS3 gene.
+|
 bp HindIII-HindIII internal deletion (305 to 492)
+|
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000040818 Scherer and Davis 1979]
 |-
+|

@@ Line 150: / Line 150: @@
 no
+|
-double mutant
+double mutant; leu2-3 and leu2-112 are each frameshift mutations; the double mutants revert very infrequently (less than 10(E-8) revertants in a culture) and cause leucine to be required for growth. This allows the selection of vectors carrying the LEU2 gene.
+|
 GTC-to-GTT silent change at codon 56,
@@ Line 163: / Line 163: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000069760 Meira LB et al., 1995];
 [http://wiki.yeastgenome.org/index.php/CommunityW303.html Rodney Rothstein, Personal communication to SGD]
 |-
+|

@@ Line 284: / Line 284: @@
+|
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=23828602 Ding et al. 2013]
 |-
+|

@@ Line 116: / Line 116: @@
 Rodney Rothstein, [http://wiki.yeastgenome.org/index.php/CommunityW303.html Personal communication to SGD]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=leu2 <em>leu2delta1</em>]
@@ Line 150: / Line 164: @@
 [http://wiki.yeastgenome.org/index.php/CommunityW303.html Rodney Rothstein, Personal communication to SGD]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=lys2 <em>lys2-801</em>]
@@ Line 176: / Line 204: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al. 1995]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=trp1 <em>trp1delta1</em>]
@@ Line 215: / Line 257: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041995 McDonald et al. 1997]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=trp1 <em>trp1-1</em>]
@@ Line 227: / Line 271: @@
+|
 Brian Green and Joachim Li, unpublished results
 |-
+|
@@ Line 255: / Line 325: @@
 |-
+|
 |}
@@ Line 261: / Line 358: @@
-{|   border="1"   class="wikitable"
+{|
 |-
 !|Allele!!Deleted ORF?!!Reverts?!!Notes !!Molecular Description<sup>a</sup>!!Reference
@@ Line 291: / Line 388: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
 |-
+|
 [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=lys2 <em>lys2delta0</em>]
@@ Line 333: / Line 431: @@
+|
 |}
 <sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
 <sup>b</sup>All trp- strains are cold sensitive ([http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057172 Singh and Manney 1974]).

@@ Line 1: / Line 1: @@
 This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications.
-<protect><!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4-->
-<!--
+{| border="1"   class="wikitable"
 |-
 !|Allele!!Deleted ORF?!!Reverts?!!Notes!!Molecular Description<sup>a</sup>!!Reference
@@ Line 260: / Line 254: @@
 Yan Li, Glenn Manthey, and Adam Bailis, unpublished results
-|- class="tableEdit_footer"
+|-
-|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4&page=28469&pagename={{FULLPAGENAMEE}}&type=0&template= edit table]</span> || || || || ||
 |}
 The alleles listed below are described in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998].
-<protect><!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.G4f59169a068f3-->
-<!--
+{|   border="1"   class="wikitable"
 |-
 !|Allele!!Deleted ORF?!!Reverts?!!Notes !!Molecular Description<sup>a</sup>!!Reference
@@ Line 343: / Line 330: @@
 [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al. 1998]
-|- class="tableEdit_footer"
+|-
-|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d001ef25e9c0a93e0c26b710b67e1344.28469.G4f59169a068f3&page=28469&pagename={{FULLPAGENAMEE}}&type=0&template= edit table]</span> || || || || ||
 |}
 <sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
 <sup>b</sup>All trp- strains are cold sensitive ([http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057172 Singh and Manney 1974]).

← Older revision		Revision as of 21:30, 8 March 2012
Line 1:		Line 1:
−	This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if ~~you have additions or modifications~~ you would like to suggest.	+	This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications.

	<protect><!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4-->		<protect><!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4-->