YIR019C

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Systematic name YIR019C
Gene name FLO11
Aliases MUC1, STA4
Feature type ORF, Verified
Coordinates Chr IX:393675..389572
Primary SGDID S000001458


Description of YIR019C: GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain[1][2][3][4][5]




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Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: REG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter

Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [6] [7]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: REG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [6] [7]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: REG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter

Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [6] [7]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: REG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [6] [7]





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References

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  1. Douglas LM, et al. (2007) Expression and Characterization of the Flocculin Flo11/Muc1, a Saccharomyces cerevisiae Mannoprotein with Homotypic Properties of Adhesion. Eukaryot Cell 6(12):2214-21 SGD PMID 17921350
  2. Guo B, et al. (2000) A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating. Proc Natl Acad Sci U S A 97(22):12158-63 SGD PMID 11027318
  3. Lambrechts MG, et al. (1996) Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast. Proc Natl Acad Sci U S A 93(16):8419-24 SGD PMID 8710886
  4. Rupp S, et al. (1999) MAP kinase and cAMP filamentation signaling pathways converge on the unusually large promoter of the yeast FLO11 gene. EMBO J 18(5):1257-69 SGD PMID 10064592
  5. Vachova L, et al. (2011) Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. J Cell Biol 194(5):679-87 SGD PMID 21875945
  6. 6.0 6.1 6.2 6.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  7. 7.0 7.1 7.2 7.3 submitted by Valmik K. Vyas on 2003-03-27

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