YCL063W

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Systematic name YCL063W
Gene name VAC17
Aliases YCL062W
Feature type ORF, Verified
Coordinates Chr III:17290..18561
Primary SGDID S000000568


Description of YCL063W: Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p[1][2][3]




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Alleles, Strains, and Phenotypes

Partial Deletion

Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable Mutation type(s): Partial deletion from 17364 to partialDelTo

In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [3] [4]


Interactions

Physical

Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [3] [4]


Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [3] [4]


Two Hybrid

Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [3] [4]


Protein Details

Protein Degradation

Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [3] [4]


Protein Function/Process

Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [3] [4]


Protein Localization

Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [3] [4]





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References

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  1. Bartholomew CR and Hardy CF (2009) p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae. Eukaryot Cell 8(4):560-72 SGD PMID 19218422
  2. Ishikawa K, et al. (2003) Identification of an organelle-specific myosin V receptor. J Cell Biol 160(6):887-97 SGD PMID 12642614
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 SGD PMID 12594460 Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content
  4. 4.0 4.1 4.2 4.3 4.4 4.5 4.6 submitted by Fusheng Tang on 2003-03-17

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