File:FY4 SRD1 story.png
Summary
Figure showing sequencing data aligned to Ensembl R64.1.100.
Original strain of FY4 (DGY1, grey track) has an undocumented gene deletion. The gene is SRD1 (YCR018C), SRD1p has a role in pre-rRNA processing and is stress associated.
Deletions observed in other lab FY4 data as well (https://doi.org/10.1093/molbev/msaa172) , ('ANC', red track).
Using long-read sequencing of DGY1657 (https://doi.org/10.1371/journal.pbio.3000069) a FY4 derived strain (DGY1657, light blue track) we find evidence of a transposon (YJR027W) intervening in the region.
RNAseq data of DGY1657 (blue track) shows that the region is being transcribed, but in the opposite direction (presumably from the bidirectional firing from the flanking CWH43 promoter). These transcripts do seem to recruit ribosomes (RPF, dark blue track) that initiate at a short ORF before presumably triggering NMD.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:10, 19 October 2021 | | 1,369 × 679 (37 KB) | Ps163 (talk | contribs) | Figure showing sequencing data aligned to Ensembl R64.1.100. Original strain of FY4 (DGY1, grey track) has an undocumented gene deletion. The gene is SRD1 (YCR018C), SRD1p has a role in pre-rRNA processing and is stress associated. Deletions observe... |
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