SGD-Wiki - User contributions [en]

SGA

2010-02-16T16:50:59Z

Yeastsoftware:

All are welcome to contribute to this page.

SGA, synthetic genetic array was developed in toronto by Boone and Andrews and their colleauges.
it aims to produce doulbe mutants in a genome wide manner for studies such as synthetic lethality and functional genomics.

'''Details of this method can be found at:'''
http://www.utoronto.ca/boonelab/pdf/SGA_protocol_final.pdf

'''Robot for this method:'''
Singer Rotor from Singer instruments at http://www.singerinst.co.uk/

'''Software for this method (scoring program):'''
YEAST-IAE at http://www.lulu.com/product/cd/sga-colony-scoring-software/5361033

Career Resources

2008-12-01T15:22:52Z

Yeastsoftware:

Following is a list of sites that post biology related job openings. Please note that this is not a comprehensive list. Some of these sites may be free (may require registration) for job seekers, job posters or both.

*[[Positions in yeast labs]] posted by members of the research community

* Yeast Bio-Products R&D Director position available through CPL Executive Search http://www.cplsearch.co.uk/search8.php

* Technical Director, Enzymes position available through CPL Executive Search http://www.cplsearch.co.uk/search29.php

*[http://genetics.faseb.org/genetics/g-gsa/open_positions.shtml Open positions] offered by [http://www.genetics-gsa.org/ Genetics Society of America]

*[http://www.ascb.org/careers/ Career Services] offered by [http://www.ascb.org/ American Society for Cell Biology]

*[http://www.healthecareers.com/site_templates/ASM/index.asp?aff=ASM&SPLD=ASM ASM Career Connections] offered by [http://www.asm.org/index.asp American Society for Microbiology]

*[http://www.faseb.org/careers/careerresources.htm Career Resources] offered by [http://www.faseb.org FASEB]

*[http://www.sdbonline.org/career/jobs.php Job Openings] offered by [http://www.sdbonline.org Society for Developmental Biology]

*[http://recruit.sciencemag.org Science Careers] and [http://nextwave.sciencemag.org Science Next Wave] offered by [http://www.scienceonline.org Science Magazine Online]

*[http://www.nature.com/naturejobs/ Nature Jobs] offered by [http://www.nature.com Nature Online]

*[http://www.biospace.com Career Center] offered by [http://www.biospace.com Biospace.com]

*[http://sciencejobs.vstore.ca SJR] Many tools to aid in search for biotech jobs. including companies and real resumes and how-to guides. all in one downloadable package.

Career Resources

2008-12-01T15:22:22Z

Yeastsoftware:

Following is a list of sites that post biology related job openings. Please note that this is not a comprehensive list. Some of these sites may be free (may require registration) for job seekers, job posters or both.

*[[Positions in yeast labs]] posted by members of the research community

* Yeast Bio-Products R&D Director position available through CPL Executive Search http://www.cplsearch.co.uk/search8.php

* Technical Director, Enzymes position available through CPL Executive Search http://www.cplsearch.co.uk/search29.php

*[http://genetics.faseb.org/genetics/g-gsa/open_positions.shtml Open positions] offered by [http://www.genetics-gsa.org/ Genetics Society of America]

*[http://www.ascb.org/careers/ Career Services] offered by [http://www.ascb.org/ American Society for Cell Biology]

*[http://www.healthecareers.com/site_templates/ASM/index.asp?aff=ASM&SPLD=ASM ASM Career Connections] offered by [http://www.asm.org/index.asp American Society for Microbiology]

*[http://www.faseb.org/careers/careerresources.htm Career Resources] offered by [http://www.faseb.org FASEB]

*[http://www.sdbonline.org/career/jobs.php Job Openings] offered by [http://www.sdbonline.org Society for Developmental Biology]

*[http://recruit.sciencemag.org Science Careers] and [http://nextwave.sciencemag.org Science Next Wave] offered by [http://www.scienceonline.org Science Magazine Online]

*[http://www.nature.com/naturejobs/ Nature Jobs] offered by [http://www.nature.com Nature Online]

*[http://www.biospace.com Career Center] offered by [http://www.biospace.com Biospace.com]

*[http://sciencejobs.vstore.ca] Many tools to aid in search for biotech jobs. including companies and real resumes and how-to guides. all in one downloadable package.

Methods

2008-12-01T15:20:12Z

Yeastsoftware:

*[http://www.fhcrc.org/labs/breeden/Methods_BreedenLab.html| Yeast Lab Protocols] from the Breeden Lab, Fred Hutchinson Cancer Research Center.

*[http://www.fhcrc.org/labs/gottschling/ Yeast Lab Protocols] from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

*[http://gasch.genetics.wisc.edu/protocols.html Yeast & Microarray Protocols] from the Gasch Lab, University of Wisconsin-Madison.

*[http://www.umanitoba.ca/faculties/medicine/biochem/gietz/Trafo.html The Definitive Yeast Transformation Homepage] from the University of Manitoba.

*[http://fangman-brewer.genetics.washington.edu/index.html DNA Replication Protocols] from the Fangman and Brewer Labs, University of Washington.

*[http://www.fccc.edu/research/labs/golemis/InteractionTrapInWork.html Interaction Trap at Work] information page from Erica Golemis's laboratory at the Fox Chase Cancer Center.

*[http://cmgm.stanford.edu/pbrown/mguide/index.html The MGuide. Version 2.0], a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.

*[http://gasch.genetics.wisc.edu/protocols/Gasch_MethEnzym.pdf Guide to Yeast Microarray Experiments], by Audrey Gasch, from <u>Guide to Yeast Genetics and Molecular and Cellular Biology</u>, Methods in Enzymology (2002)

*The [http://depts.washington.edu/yeastrc/ NCRR Yeast Resource Center] at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.

*Full text of the paper (Erdeniz <i>et al.</i> (1997) Genome Res. 7:1174-1183) describing [http://www.genome.org/cgi/content/full/7/12/1174 PCR-Based Allele Replacement]. Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein.

*[http://genomics.princeton.edu/dunham/chemostat.html Chemostat Manual] from Maitreya Dunham's lab, Princeton University. See her [http://genomics.princeton.edu/dunham/protocols.shtml other protocols].

*[http://www.phys.ksu.edu/gene/chapters.html A Classroom Guide To Yeast Experiments] from The GENE project at Kansas State University.

*[http://openwetware.org/wiki/The_mRNA_Decay_Resource:Protocols_and_Resources Protocols and Resources] from the RNA Decay Resource, a resource for the sharing of information, know-how, and wisdom among researchers who study mRNA decay.

*[[SGA]] SGA protocols and scoring software.

SGA

2008-12-01T15:06:56Z

Yeastsoftware: first post of SGA