SGD-Wiki - User contributions [en]

Methods

2018-05-03T22:13:43Z

Jhouseley:

*[http://research.fhcrc.org/breeden/en/methods.html Yeast Lab Protocols] from the Breeden Lab, Fred Hutchinson Cancer Research Center.

*[http://research.fhcrc.org/gottschling/en/protocols/yeast-protocols.html Yeast Lab Protocols] from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

*[http://gasch.genetics.wisc.edu/protocols.html Yeast & Microarray Protocols] from the Gasch Lab, University of Wisconsin-Madison.

*[http://home.cc.umanitoba.ca/~gietz/ The Definitive Yeast Transformation Homepage] from the University of Manitoba.

*[http://fangman-brewer.genetics.washington.edu/index.html DNA Replication Protocols] from the Fangman and Brewer Labs, University of Washington.

*[http://cmgm.stanford.edu/pbrown/mguide/index.html The MGuide. Version 2.0], a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.

*[http://gasch.genetics.wisc.edu/protocols/Gasch_MethEnzym.pdf Guide to Yeast Microarray Experiments], by Audrey Gasch, from Guide to Yeast Genetics and Molecular and Cellular Biology, Methods in Enzymology (2002)

*[http://depts.washington.edu/yeastrc/ The NCRR Yeast Resource Center] at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.

* [http://genome.cshlp.org/content/7/12/1174.full PCR-Based Allele Replacement] (Erdeniz et al. (1997) Genome Res. 7:1174-1183). Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bärtsch on the W303 strain; derivatives were used in the study'').

*[http://dunham.gs.washington.edu/home.shtml Chemostat Manual] from Maitreya Dunham's lab, University of Washington.

*[http://www.phys.ksu.edu/gene/chapters.html A Classroom Guide To Yeast Experiments] from The GENE project at Kansas State University based on [http://www.rushessay.com essay writing].

*[http://openwetware.org/wiki/The_mRNA_Decay_Resource:Protocols_and_Resources Protocols and Resources] from the RNA Decay Resource, a resource for the sharing of information, know-how, and wisdom among researchers who study mRNA decay.

*[[SGA]] protocols and scoring software.

*[http://Vadlo.com/ Molecular Biology Protocols Search Engine] from Life in Research, LLC. Also life sciences databases, online tools, software, and powerpoints. For example [http://www.vadlo.com/b/q?k=Yeast+Protocols&rel=0 Yeast protocols], [http://www.vadlo.com/b/q?k=Yeast&rel=3 Yeast databases]

*[http://wiki.yeastgenome.org/index.php/Software Software tools] created by members of the scientific community.
*[http://h.web.umkc.edu/honigbergs/yeastcommunity/concept.html.html Yeast Community Site] from Honigberg Lab, University of Missouri Kansas City.

*[http://www.magusto.com/ Concours Site] from concours Lab, University of Mousseaux sur seine (protocol in French).

*[[Yeast_Cell_Micromanipulation|Yeast Cell Micromanipulation]] Protocol for Making Support Rod with Fiber Optic Needle Attached from Cora Styles

*[http://yeastgenome.org/cgi-bin/geneticData/displayTwoPoint? Genetic Mapping (Two-Point) Data] for yeast genes.
*[[GRSandPRIMED|PRIMED]]: Complete primer set for deleting and C-terminally tagging every protein-coding and non-coding RNA gene in ''S. cerevisiae''.

*[http://www.babraham.ac.uk/our-research/epigenetics/jon-houseley/protocols Yeast Protocols] particularly DNA/RNA analysis, from the Houseley Lab at the Babraham Institute. Updated May 2018.

Positions in yeast labs

2016-02-22T22:00:23Z

Jhouseley:

=='''Postdoctoral position in evolutionary systems biology. Québec, Canada. (posted Feb 23, 2016)''' ==
'''Postdoctoral position in evolutionary genomics and systems biology in the Landry Laboratory'''

A postdoctoral position is available in the Landry Laboratory at Université Laval in Québec City under the Canada Research Chair in Evolutionary Cell and Systems Biology. The PDF will work on a project at the interface of genomics, cell biology and evolution to investigate the mechanisms of evolution of gene and protein networks. The specific project will be developed with the selected candidate. The selected candidate will combine experimental evolution, high-throughput screening and bioinformatics, and the budding yeast Saccharomyces cerevisiae as experimental model system. The candidate is expected to have a PhD in biology or a related discipline, and a strong background in molecular and cell biology with at least basic skills in bioinformatics and statistics (R, Python or Perl). The candidate should have strong leadership skills, motivation and creativity and be able to work in a team of collaborators. The Landry lab is located at the Institut de Biologie Intégrative et des Systèmes (IBIS) of Université Laval and is part of the Quebec Network for Research on Protein Function, Engineering, and Applications (PROTEO). IBIS and PROTEO offer very stimulating training environment and cutting edge technologies in genomics and proteomics. The Landry lab is an international team of 15 students, PDFs and research associates from different backgrounds (microbiology, biology, bioinformatics, biochemistry) addressing questions in evolutionary cell and systems biology.

The application package (1 single PDF file) should include a motivation letter demonstrating the interest of the candidate for the field and his/her ability to perform this type of research, a short project proposal (half a page), reprints of the candidate’s most important contributions, a CV and the contact information of three people who can provide letters of reference. The file should be sent to landrylaboratory@gmail.com

Starting date could be as early as June 2016. The competition will remain open until a candidate is selected.
For recent publications from the Landry lab, please visit:
http://landrylab.ibis.ulaval.ca/

=='''Postdoctoral position in genetics, evolution and virulence of yeasts. Dublin, Ireland (posted Feb 12, 2016)''' ==
Laboratories of Ken Wolfe and Geraldine Butler, University College Dublin, Ireland

Three postdoctoral positions are available in the Wolfe and Butler labs.

Position 1: Analysis of virulence in the pathogenic yeast ''Candida parapsilosis'' (one early stage postdoctoral position).

Positions 2 and 3: Genetics and evolution in Biotech Yeasts (two positions, at early or senior post-doctoral level).

For full details, see http://www.nature.com/naturejobs/science/jobs/572287-genetics-evolution-and-virulence-of-yeasts

Closing date for applications: March 4, 2016.

Contact: Ken Wolfe, kenneth.wolfe@ucd.ie

=='''Postdoctoral position in Cell Biology and Genetics, Northern Kentucky University (posted May 7, 2015)''' ==
Laboratory of Erin Strome, Biological Sciences Department, Northern Kentucky University (Cincinnati Area)

A teaching-scholar faculty position is available to study mechanisms of haploinsufficiency induced genome instability. The position would be ideal for someone who will be pursuing a teaching and research balanced career and potentially be interested in a faculty job at an undergraduate institution. The postdoctoral fellow will have opportunities to develop their experimental and scientific credentials while also getting teaching experience and mentoring on teaching and lots of direct contact mentoring undergraduates in research lab projects.

Qualifications: Applicants should have a Ph.D. (ABD candidates will be considered) with a strong background in molecular biology/biochemistry/genetics and should be capable of conducting standard molecular biology tests including PCR and qPCR, Westerns, and siRNA experiments.

Please see https://jobs.nku.edu/postings/2817 for full job ad and application details.

Contact: Erin Strome, stromee1@nku.edu

Methods

2015-09-23T13:34:18Z

Jhouseley:

*[http://labs.fhcrc.org/breeden/Methods/index.html Yeast Lab Protocols] from the Breeden Lab, Fred Hutchinson Cancer Research Center.

*[http://research.fhcrc.org/gottschling/en/protocols/yeast-protocols.html Yeast Lab Protocols] from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

*[http://gasch.genetics.wisc.edu/protocols.html Yeast & Microarray Protocols] from the Gasch Lab, University of Wisconsin-Madison.

*[http://home.cc.umanitoba.ca/~gietz/ The Definitive Yeast Transformation Homepage] from the University of Manitoba.

*[http://fangman-brewer.genetics.washington.edu/index.html DNA Replication Protocols] from the Fangman and Brewer Labs, University of Washington.

*[http://cmgm.stanford.edu/pbrown/mguide/index.html The MGuide. Version 2.0], a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.

*[http://gasch.genetics.wisc.edu/protocols/Gasch_MethEnzym.pdf Guide to Yeast Microarray Experiments], by Audrey Gasch, from Guide to Yeast Genetics and Molecular and Cellular Biology, Methods in Enzymology (2002)

*[http://depts.washington.edu/yeastrc/ The NCRR Yeast Resource Center] at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.

* [http://genome.cshlp.org/content/7/12/1174.full PCR-Based Allele Replacement] (Erdeniz et al. (1997) Genome Res. 7:1174-1183). Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bärtsch on the W303 strain; derivatives were used in the study'').

*[http://dunham.gs.washington.edu/home.shtml Chemostat Manual] from Maitreya Dunham's lab, University of Washington.

*[http://www.phys.ksu.edu/gene/chapters.html A Classroom Guide To Yeast Experiments] from The GENE project at Kansas State University based on [http://www.rushessay.com essay writing].

*[http://openwetware.org/wiki/The_mRNA_Decay_Resource:Protocols_and_Resources Protocols and Resources] from the RNA Decay Resource, a resource for the sharing of information, know-how, and wisdom among researchers who study mRNA decay.

*[[SGA]] protocols and scoring software.

*[http://Vadlo.com/ Molecular Biology Protocols Search Engine] from Life in Research, LLC. Also life sciences databases, online tools, software, and powerpoints. For example [http://www.vadlo.com/b/q?k=Yeast+Protocols&rel=0 Yeast protocols], [http://www.vadlo.com/b/q?k=Yeast&rel=3 Yeast databases]

*[http://wiki.yeastgenome.org/index.php/Software Software tools] created by members of the scientific community.
*[http://sbs.umkc.edu/yeastcommunity/ Yeast Community Site] from Honigberg Lab, University of Missouri Kansas City.

*[http://www.magusto.com/ Concours Site] from concours Lab, University of Mousseaux sur seine (protocol in French).

*[[Yeast_Cell_Micromanipulation|Yeast Cell Micromanipulation]] Protocol for Making Support Rod with Fiber Optic Needle Attached from Cora Styles

*[http://yeastgenome.org/cgi-bin/geneticData/displayTwoPoint? Genetic Mapping (Two-Point) Data] for yeast genes.
*[[GRSandPRIMED|PRIMED]]: Complete primer set for deleting and C-terminally tagging every protein-coding and non-coding RNA gene in ''S. cerevisiae''.

*[http://www.babraham.ac.uk/our-research/epigenetics/jon-houseley/protocols Yeast Protocols] particularly DNA/RNA analysis, from the Houseley Lab at the Babraham Institute.