Microarray methods for profiling the Yeast Knockout (YKO) Collection

2009-03-04T15:16:05Z

Jcmatese: New page: == Intro == This page is intended to be a resource for those researchers who are interested in applying microarray-based assays to the [http://www-sequence.stanford.edu/group/yeast_deleti...

== Intro ==

This page is intended to be a resource for those researchers who are interested in applying microarray-based assays to the [http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html Yeast Knockout (YKO) Collection].
Please post links to protocols, available array platforms, and publications.

== Protocol Links ==

== Array Platforms ==

== Publications ==
*[http://www.springerlink.com/index/q0706680x2100402.pdf Zhang et al. Using the yeast genome-wide gene-deletion collection for systematic genetic screens. Methods Mol Biol (2004) vol. 241 pp. 143-61]

*[http://nar.oxfordjournals.org/cgi/content/full/33/12/e103 Yuan et al. Improved microarray methods for profiling the Yeast Knockout strain collection. Nucleic Acids Res (2005) vol. 33 (12) pp. e103]

*[http://www.nature.com/nprot/journal/v2/n11/abs/nprot.2007.427.html Pierce et al. Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures. Nat Protoc (2007) vol. 2 (11) pp. 2958-74]

Reagents

2009-03-04T14:42:18Z

Jcmatese:

*[[Antibodies]] made to ''S. cerevisiae'' proteins

*Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
**[http://www.geneworks.com.au/Content.aspx?p=84 GeneWorks]
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*[http://www.openbiosystems.com/GeneExpression/Yeast/ORF/ MORF collection] is a moveable ORF library which enables protein expression and purification for more than 4900 S. cerevisiae genes, as described in [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000113921 Gelperin DM, et al. (2005)].
**[http://wiki.yeastgenome.org/index.php/Reagents/MORF_comments Comments on MORF collection]

*[http://www.addgene.org/yeast_gateway/ pAG Vectors] is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository [http://www.addgene.org/ Addgene]. Clones were created by the [http://web.wi.mit.edu/lindquist/pub/ Lindquist Lab] at the [http://www.wi.mit.edu/ Whitehead Institute]. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. Yeast. In Press.

*The [http://plasmid.med.harvard.edu/PLASMID/ PlasmID] repository distributes over 5,200 fully sequence-verified S. cerevisiae genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the [http://www.hip.harvard.edu Harvard Institute of Proteomics] at [http://hms.harvard.edu Harvard Medical School].

*[http://www.tritechresearch.com/cgi-bin/shop/yeast.html Tritech Research] maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.

*[http://seq.yeastgenome.org/vectordb/ VectorDB], collected sequences of cloning vectors for use in a variety of organisms

*[[Microarray methods for profiling the Yeast Knockout (YKO) Collection]]

SGD-Wiki - User contributions [en]

Microarray methods for profiling the Yeast Knockout (YKO) Collection

Reagents