SGD-Wiki - User contributions [en]

YHR007C

2011-07-13T19:26:45Z

Chandralt4: Erg11 regulator

{{PageTop}}
<protect>
{|{{Prettytable}} align = 'right' width = '200px'
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000001049 YHR007C]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''ERG11 ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Aliases''' ||''CYP51''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Feature type''' || ORF, Verified[[Category:ORF]][[Category:ORF, Verified]]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr VIII:121683..120091
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000001049
|}
 
'''Description of YHR007C:''' Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family<ref name='S000057081'>Kalb VF, et al. (1987) Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6(6):529-37 {{SGDpaper|S000057081}} PMID 3322742</ref><ref name='S000058496'>Paltauf F, et al. (1992) Regulation and compartmentalization of lipid synthesis in yeast. Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press {{SGDpaper|S000058496}} PMID </ref><ref name='S000039432'>Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30 {{SGDpaper|S000039432}} PMID 7791530</ref><ref name='S000047307'>Truan G, et al. (1994) Cloning and characterization of a yeast cytochrome b5-encoding gene which suppresses ketoconazole hypersensitivity in a NADPH-P-450 reductase-deficient strain. Gene 142(1):123-7 {{SGDpaper|S000047307}} PMID 8181746</ref><ref name='S000057121'>Yoshida Y and Aoyama Y (1987) Interaction of azole antifungal agents with cytochrome P-45014DM purified from Saccharomyces cerevisiae microsomes. Biochem Pharmacol 36(2):229-35
{{SGDpaper|S000057121}} PMID 3545213</ref>
 
 
 
 
 
</protect>
__TOC__
==Community Commentary==
{{CommentaryHelp}}

NCP1 encodes the reductase that acts on Erg11, lanosterol demethylase




<protect>

==References==

{{RefHelp}}
</protect>

YJL156C

2008-09-23T17:00:23Z

Chandralt4: /* Community Commentary */

{{PageTop}}
<protect>
{|{{Prettytable}} align = 'right' width = '200px'
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000003692 YJL156C]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''SSY5 ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Aliases''' ||'' ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Feature type''' || ORF, Verified[[Category:ORF]][[Category:ORF, Verified]]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr X:128949..126886
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000003692
|}
 
'''Description of YJL156C:''' Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes<ref name='S000114586'>Poulsen P, et al. (2006) Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 5(3):601-8 {{SGDpaper|S000114586}} PMID 16524914</ref><ref name='S000066134'>Forsberg H, et al. (2001) The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids. Mol Microbiol 42(1):215-28 {{SGDpaper|S000066134}} PMID 11679080</ref><ref name='S000059760'>Forsberg H and Ljungdahl PO (2001) Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21(3):814-26
{{SGDpaper|S000059760}} PMID 11154269</ref>
 
 
 
 
 
</protect>
__TOC__
==Community Commentary==
{{CommentaryHelp}}

The Fungal Alignment Viewer at SGD indicates that SSY5/YJL156C in S. cerevisiae contains a frame-shift in the 4th-to-last codon (addition of an A), thereby truncating the protein relative to the other sensu stricto strains. In the other strains the ORF is a dozen amino acids longer. The protein and DNA sequences are highly conserved in this region, which contains a serine protease domain. There are a couple of possibilities. 
1) The reference genome S288C may contain this sequence and the gene is in fact shorter (687 amino acids). 
2) The reference sequence for S288C is wrong. Only sequencing of the reference strain can tell.
 
This difference is cited in: Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell. 2005 Jun;4(6):1116-24. PMID: 15947203 In this strain, the SSY5 ORF is 12 amino acids longer than the sequence in SGD.

Update: Option 2 has been confirmed. The additional A is erroneous and SSY5 is indeed 699 amino acids long. SGD plans to update SSY5 sequence in December, 2008.




<protect>

==References==

{{RefHelp}}
</protect>

YJL156C

2008-09-23T16:59:52Z

Chandralt4: /* Community Commentary */

{{PageTop}}
<protect>
{|{{Prettytable}} align = 'right' width = '200px'
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000003692 YJL156C]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''SSY5 ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Aliases''' ||'' ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Feature type''' || ORF, Verified[[Category:ORF]][[Category:ORF, Verified]]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr X:128949..126886
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000003692
|}
 
'''Description of YJL156C:''' Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes<ref name='S000114586'>Poulsen P, et al. (2006) Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 5(3):601-8 {{SGDpaper|S000114586}} PMID 16524914</ref><ref name='S000066134'>Forsberg H, et al. (2001) The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids. Mol Microbiol 42(1):215-28 {{SGDpaper|S000066134}} PMID 11679080</ref><ref name='S000059760'>Forsberg H and Ljungdahl PO (2001) Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21(3):814-26
{{SGDpaper|S000059760}} PMID 11154269</ref>
 
 
 
 
 
</protect>
__TOC__
==Community Commentary==
{{CommentaryHelp}}

The Fungal Alignment Viewer at SGD indicates that SSY5/YJL156C in S. cerevisiae contains a frame-shift in the 4th-to-last codon (addition of an A), thereby truncating the protein relative to the other sensu stricto strains. In the other strains the ORF is a dozen amino acids longer. The protein and DNA sequences are highly conserved in this region, which contains a serine protease domain. There are a couple of possibilities. 
1) The reference genome S288C may contain this sequence and the gene is in fact shorter (687 amino acids). 
2) The reference sequence for S288C is wrong. Only sequencing of the reference strain can tell.
 
This difference is cited in: Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell. 2005 Jun;4(6):1116-24. PMID: 15947203 In this strain, the SSY5 ORF is 12 amino acids longer than the sequence in SGD.

Update: Option 2 has been confirmed. The additional A is erroneous and SSY5 is indeed 699 amino acids long. SGD plans to update SSY5 sequence in December.




<protect>

==References==

{{RefHelp}}
</protect>

YJL156C

2008-09-22T17:47:55Z

Chandralt4: /* Community Commentary */

{{PageTop}}
<protect>
{|{{Prettytable}} align = 'right' width = '200px'
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000003692 YJL156C]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''SSY5 ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Aliases''' ||'' ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Feature type''' || ORF, Verified[[Category:ORF]][[Category:ORF, Verified]]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr X:128949..126886
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000003692
|}
 
'''Description of YJL156C:''' Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes<ref name='S000114586'>Poulsen P, et al. (2006) Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 5(3):601-8 {{SGDpaper|S000114586}} PMID 16524914</ref><ref name='S000066134'>Forsberg H, et al. (2001) The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids. Mol Microbiol 42(1):215-28 {{SGDpaper|S000066134}} PMID 11679080</ref><ref name='S000059760'>Forsberg H and Ljungdahl PO (2001) Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21(3):814-26
{{SGDpaper|S000059760}} PMID 11154269</ref>
 
 
 
 
 
</protect>
__TOC__
==Community Commentary==
{{CommentaryHelp}}

The Fungal Alignment Viewer at SGD indicates that SSY5/YJL156C in S. cerevisiae contains a frame-shift in the 4th-to-last codon (addition of an A), thereby truncating the protein relative to the other sensu stricto strains. In the other strains the ORF is a dozen amino acids longer. The protein and DNA sequences are highly conserved in this region, which contains a serine protease domain. There are a couple of possibilities. 
1) The reference genome S288C may contain this sequence and the gene is in fact shorter. 
2) The reference sequence for S288C is wrong. Only sequencing of the reference strain can tell.
 
This difference is cited in: Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell. 2005 Jun;4(6):1116-24. PMID: 15947203 In this strain, the SSY5 ORF is 12 amino acids longer than the sequence in SGD.




<protect>

==References==

{{RefHelp}}
</protect>

YJL156C

2008-09-22T17:33:16Z

Chandralt4: /* Community Commentary */

{{PageTop}}
<protect>
{|{{Prettytable}} align = 'right' width = '200px'
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000003692 YJL156C]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''SSY5 ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Aliases''' ||'' ''
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Feature type''' || ORF, Verified[[Category:ORF]][[Category:ORF, Verified]]
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr X:128949..126886
|-
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000003692
|}
 
'''Description of YJL156C:''' Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes<ref name='S000114586'>Poulsen P, et al. (2006) Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 5(3):601-8 {{SGDpaper|S000114586}} PMID 16524914</ref><ref name='S000066134'>Forsberg H, et al. (2001) The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids. Mol Microbiol 42(1):215-28 {{SGDpaper|S000066134}} PMID 11679080</ref><ref name='S000059760'>Forsberg H and Ljungdahl PO (2001) Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21(3):814-26
{{SGDpaper|S000059760}} PMID 11154269</ref>
 
 
 
 
 
</protect>
__TOC__
==Community Commentary==
{{CommentaryHelp}}

The Fungal Alignment Viewer at SGD indicates that SSY5/YJL156C in S. cerevisiae contains a frame-shift in the 4th-to-last codon (addition of an A), thereby truncating the protein relative to the other sensu stricto strains. In the other strains the ORF is a dozen amino acids longer. The protein and DNA sequences are highly conserved in this region, which contains a serine protease domain. There are a couple of possibilities.
1) The reference genome S288C may contain this sequence and the gene is in fact shorter.
2) The reference sequence for S288C is wrong. Only sequencing of the reference strain can tell.




<protect>

==References==

{{RefHelp}}
</protect>