Reagents

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*[[Antibodies]] made to ''S. cerevisiae'' proteins
 
*[[Antibodies]] made to ''S. cerevisiae'' proteins
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*[http://www.corastyles.com/ Cora Styles Lab Supplies] - Simple, high-quality yeast dissection and replica plating tools, including yeast dissection needle kits, velveteens, and replica plating blocks.
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*Oligos and Primers
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**[http://wiki.yeastgenome.org/index.php/Primer_Set_Sequences Primer Set Sequences] Links to download the sequences of primer sets from ResGen/Invitrogen and from the Yeast Knockout (YKO) Consortium
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**Several companies provide custom oligo synthesis, including but not limited to, those listed here:
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***[http://www.geneworks.com.au/Content.aspx?p=84 GeneWorks]
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***[http://www.idtdna.com/pages/products?adwords=oligo&gclid=CMT0tsS9_pMCFRwvagodWhd3WA Integrated DNA Technologies]
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***[http://www.invitrogen.com/site/us/en/home/brands/Invitrogen.html Invitrogen]
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***Find other companies by searching [http://www.google.com/search?client=safari&rls=en&q=order+oligos+primers&ie=UTF-8&oe=UTF-8 Google]
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*[http://www.thermoscientificbio.com/non-mammalian-cdna-and-orf/yeast-orf-collection/ MORF collection] is a moveable ORF library which enables protein expression and purification for more than 4900 <i>S. cerevisiae</i> genes, as described in [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000113921 Gelperin DM, et al.  (2005)].
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*[http://www.tritechresearch.com/index.html?research_type=yeast Tritech Research] maintains a list of links to <i>S. cerevisiae</i> information and protocols, and sells reagents and equipment for <i>S. cerevisiae</i> research.
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*[[Microarray methods for profiling the Yeast Knockout (YKO) Collection]]
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*[http://www.sunrisescience.com/ Yeast Growth Media] - Sunrise Science Products manufactures hundreds of selective and non-selective yeast media formulations, and custom recipes for yeast and other organisms can be quickly made to your specification.
  
*Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
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==Vectors==
**[http://www.geneworks.com.au/Content.aspx?p=84 GeneWorks]
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*[http://www.addgene.org/ Addgene]: A Better Way to Share PlasmidsAddgene is a non-profit plasmid repository dedicated to helping academic scientists around the world share high-quality plasmids.
**[http://www.idtdna.com/catalog/catalog.aspx?adwords=oligo&gclid=CMT0tsS9_pMCFRwvagodWhd3WA Integrated DNA Technologies]
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**[http://www.invitrogen.com.cn/content.cfm?pageid=9714 Invitrogen]
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**Find other companies by searching [http://www.google.com/search?client=safari&rls=en&q=order+oligos+primers&ie=UTF-8&oe=UTF-8 Google]
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*[http://www.openbiosystems.com/GeneExpression/Yeast/ORF/ MORF collection] is a moveable ORF library which enables protein expression and purification for more than 4900 <i>S. cerevisiae</i> genes, as described in [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000113921 Gelperin DM, et al(2005)].
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**[http://wiki.yeastgenome.org/index.php/Reagents/MORF_comments Comments on MORF collection]
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*[http://www.addgene.org/yeast_gateway/ pAG Vectors] is a collection of 288 yeast Gateway expression vectors. These "<u>A</u>dvanced <u>G</u>ateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository [http://www.addgene.org/ Addgene]. Clones were created by the [http://web.wi.mit.edu/lindquist/pub/ Lindquist Lab] at the [http://www.wi.mit.edu/ Whitehead Institute]. "A suite of Gateway vectors for high-throughput genetic analysis in <i>Saccharomyces cerevisiae</i>". Simon Alberti, Aaron D. Gitler, and Susan Lindquist. 2007. <i>Yeast</i>. In Press.
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*The [http://plasmid.med.harvard.edu/PLASMID/ PlasmID] repository distributes over 5,200 fully sequence-verified <i>S. cerevisiae</i> genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the [http://www.hip.harvard.edu Harvard Institute of Proteomics] at [http://hms.harvard.edu Harvard Medical School].
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*The [http://dnasu.asu.edu/DNASU/Home.jsp DNASU Plasmid Repository] stores and distributes nearly 100,000 plasmids including over 35,000 human and mouse plasmids, full genome collections of numerous organisms including <i>S. cerevisiae</i>, the protein expression plasmids from the Protein Structure Initiative Material Repository (PSI-MR), and both small and large collections from individual researchers. DNASU accepts plasmid contributions from individual researchers and will distribute them to the community.
 
*The [http://dnasu.asu.edu/DNASU/Home.jsp DNASU Plasmid Repository] stores and distributes nearly 100,000 plasmids including over 35,000 human and mouse plasmids, full genome collections of numerous organisms including <i>S. cerevisiae</i>, the protein expression plasmids from the Protein Structure Initiative Material Repository (PSI-MR), and both small and large collections from individual researchers. DNASU accepts plasmid contributions from individual researchers and will distribute them to the community.
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*[http://www.addgene.org/yeast-gateway/ pAG Vectors] is a collection of 288 yeast Gateway expression vectors. These "<u>A</u>dvanced <u>G</u>ateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository [http://www.addgene.org/ Addgene]. Clones were created by the [http://lindquistlab.wi.mit.edu/ Lindquist Lab] at the [http://wi.mit.edu/ Whitehead Institute]. "A suite of Gateway vectors for high-throughput genetic analysis in <i>Saccharomyces cerevisiae</i>". Simon Alberti, Aaron D. Gitler, and Susan Lindquist. 2007. <i>Yeast</i>.
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*The [http://plasmid.med.harvard.edu/PLASMID/ PlasmID] repository distributes over 5,200 fully sequence-verified <i>S. cerevisiae</i> genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the [http://harlow.hms.harvard.edu/ Harvard Institute of Proteomics] at [http://hms.harvard.edu/ Harvard Medical School].
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*[http://genome-www.stanford.edu/vectordb/ VectorDB], collected sequences of cloning vectors for use in a variety of organisms.
  
*[http://www.tritechresearch.com/cgi-bin/shop/yeast.html Tritech Research] maintains a list of links to <i>S. cerevisiae</i> information and protocols, and sells reagents and equipment for <i>S. cerevisiae</i> research.
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*[[Yeastriction|CRISPR]] plasmids and yeast strains designed to help with CRISPR genome editing
 
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*[http://seq.yeastgenome.org/vectordb/ VectorDB], collected sequences of cloning vectors for use in a variety of organisms
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*[[Microarray methods for profiling the Yeast Knockout (YKO) Collection]]
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Latest revision as of 20:07, 25 March 2015

[edit] Vectors

  • Addgene: A Better Way to Share Plasmids. Addgene is a non-profit plasmid repository dedicated to helping academic scientists around the world share high-quality plasmids.
  • The DNASU Plasmid Repository stores and distributes nearly 100,000 plasmids including over 35,000 human and mouse plasmids, full genome collections of numerous organisms including S. cerevisiae, the protein expression plasmids from the Protein Structure Initiative Material Repository (PSI-MR), and both small and large collections from individual researchers. DNASU accepts plasmid contributions from individual researchers and will distribute them to the community.
  • pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository Addgene. Clones were created by the Lindquist Lab at the Whitehead Institute. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist. 2007. Yeast.
  • The PlasmID repository distributes over 5,200 fully sequence-verified S. cerevisiae genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the Harvard Institute of Proteomics at Harvard Medical School.
  • VectorDB, collected sequences of cloning vectors for use in a variety of organisms.
  • CRISPR plasmids and yeast strains designed to help with CRISPR genome editing
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