Difference between revisions of "GRSandPRIMED"
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[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome. | [http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome. | ||
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Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files. | Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files. | ||
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The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper]. | The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper]. | ||
Revision as of 13:27, 5 February 2015
Genome Retrieval Script (GRS) and PRIMED
Genome Retrieval Script (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.
Using GRS, the authors created PRIMED: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.
The publication describing the software and the primers it generated are described in a PLOS ONE paper.
