Difference between revisions of "Commonly used strains"

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This page describes some of the most commonly used yeast lab strains. Much of the information is taken from [http://www.urmc.rochester.edu/biochemistry-biophysics/images/Getting-Started-With-Yeast.pdf F. Sherman (2002)] Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:
 
This page describes some of the most commonly used yeast lab strains. Much of the information is taken from [http://www.urmc.rochester.edu/biochemistry-biophysics/images/Getting-Started-With-Yeast.pdf F. Sherman (2002)] Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:
  
* [http://db.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston] (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
+
* [http://www.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston] (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
* [http://db.yeastgenome.org/reference/S000079648/overview van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
+
* [http://www.yeastgenome.org/reference/S000079648/overview van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
* [http://db.yeastgenome.org/reference/S000080159/overview Winzeler et al.] (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains
+
* [http://www.yeastgenome.org/reference/S000080159/overview Winzeler et al.] (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains
  
  
Line 13: Line 13:
 
The S288C genome was recently resequenced at the [http://www.sanger.ac.uk/research/projects/genomeinformatics/ Sanger Institute].
 
The S288C genome was recently resequenced at the [http://www.sanger.ac.uk/research/projects/genomeinformatics/ Sanger Institute].
  
'''References:''' [http://db.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston] (1986) Genetics 113:35-43.
+
'''References:''' [http://www.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston] (1986) Genetics 113:35-43.
  
 
'''Sources:''' [http://www.atcc.org/Products/All/204508.aspx ATCC:204508]
 
'''Sources:''' [http://www.atcc.org/Products/All/204508.aspx ATCC:204508]
Line 20: Line 20:
 
'''Genotype:''' ''MAT''a/α ''his3''Δ''1/his3''Δ''1 leu2''Δ''0/leu2''Δ''0 LYS2/lys2''Δ''0 met15''Δ''0/MET15 ura3''Δ''0/ura3''Δ''0''
 
'''Genotype:''' ''MAT''a/α ''his3''Δ''1/his3''Δ''1 leu2''Δ''0/leu2''Δ''0 LYS2/lys2''Δ''0 met15''Δ''0/MET15 ura3''Δ''0/ura3''Δ''0''
  
'''Notes:''' Strain used in the [http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. As in S288c, this strain as well as haploid derivatives BY4741, and BY4742 have allelic variants of [http://www.yeastgenome.org/locus/MIP1/overview ''MIP1''],  [http://www.yeastgenome.org/locus/SAL1/overview ''SAL1''] and [http://www.yeastgenome.org/locus/CAT5/overview ''CAT5''] and these polymorphisms, described in the respective locus history notes for these genes ([http://www.yeastgenome.org/locus/MIP1/overview#history ''MIP1''],  [http://www.yeastgenome.org/locus/SAL1/overview#history ''SAL1''] and [http://www.yeastgenome.org/cgi-bin/locusHistory.pl?dbid=S000005651 ''CAT5''])  all contribute to the high observed petite frequency. Details regarding the contributions of these variants to petite formation are referenced in [http://www.yeastgenome.org/reference/S000130847/overview Dimitrov et al.] (2009) Genetics 183(1):365-83. See the Brachmann et al., 1998 reference for details of strain construction.
+
'''Notes:''' Strain used in the [http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. As in S288c, this strain as well as haploid derivatives BY4741, and BY4742 have allelic variants of [http://www.yeastgenome.org/locus/MIP1/overview ''MIP1''],  [http://www.yeastgenome.org/locus/SAL1/overview ''SAL1''] and [http://www.yeastgenome.org/locus/CAT5/overview ''CAT5''] and these polymorphisms, described in the respective locus history notes for these genes ([http://www.yeastgenome.org/locus/S000005857/sequence#history ''MIP1''],  [http://www.yeastgenome.org/locus/S000005027/sequence#history ''SAL1''] and [http://www.yeastgenome.org/locus/cat5/sequence#history ''CAT5''])  all contribute to the high observed petite frequency. Details regarding the contributions of these variants to petite formation are referenced in [http://www.yeastgenome.org/reference/S000130847/overview Dimitrov et al.] (2009) Genetics 183(1):365-83. See the Brachmann et al., 1998 reference for details of strain construction.
  
'''References:''' [http://db.yeastgenome.org/reference/S000041186/overview Brachmann et al.] (1998) Yeast 14:115-32.
+
'''References:''' [http://www.yeastgenome.org/reference/S000041186/overview Brachmann et al.] (1998) Yeast 14:115-32.
  
'''Sources:''' [http://www.thermoscientificbio.com/search/?term=YSC1050 Thermo Scientific:YSC1050]
+
'''Sources:''' [http://www.atcc.org/products/all/201390.aspx ATCC:201390]
  
 
==FY4==
 
==FY4==
Line 31: Line 31:
 
'''Notes:''' Derived from S288C.
 
'''Notes:''' Derived from S288C.
  
'''References:'''  [http://db.yeastgenome.org/reference/S000047446/overview Winston et al.] (1995) Yeast 11:53-55.
+
'''References:'''  [http://www.yeastgenome.org/reference/S000047446/overview Winston et al.] (1995) Yeast 11:53-55.
  
[http://db.yeastgenome.org/reference/9483801/overview Brachmann et al.] (1998) Yeast 14:115-32.
+
[http://www.yeastgenome.org/reference/9483801/overview Brachmann et al.] (1998) Yeast 14:115-32.
  
 
===DBY12020===
 
===DBY12020===
Line 54: Line 54:
 
'''Notes:'''  Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
'''Notes:'''  Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
  
'''References:''' [http://db.yeastgenome.org/reference/S000047446/overview Winston et al.] (1995) Yeast 11:53-55.
+
'''References:''' [http://www.yeastgenome.org/reference/S000047446/overview Winston et al.] (1995) Yeast 11:53-55.
  
 
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF:10000D]
 
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF:10000D]
Line 63: Line 63:
 
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-''trp1''.
 
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-''trp1''.
  
'''References:''' [http://db.yeastgenome.org/reference/S000057090/overview Olson MV et al.] (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.
+
'''References:''' [http://www.yeastgenome.org/reference/S000057090/overview Olson MV et al.] (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.
  
 
'''Sources:''' [http://www.atcc.org/Products/All/204511.aspx ATCC:204511]
 
'''Sources:''' [http://www.atcc.org/Products/All/204511.aspx ATCC:204511]
Line 72: Line 72:
 
'''Notes:''' Used in the systematic sequencing project, the sequence stored in SGD.
 
'''Notes:''' Used in the systematic sequencing project, the sequence stored in SGD.
  
'''References:''' [http://db.yeastgenome.org/reference/S000079649/overview Hartwell] (1967) J. Bacteriol. 93:1662-1670.
+
'''References:''' [http://www.yeastgenome.org/reference/S000079649/overview Hartwell] (1967) J. Bacteriol. 93:1662-1670.
  
 
'''Sources:''' [http://www.atcc.org/Products/All/208526.aspx ATCC:208526]
 
'''Sources:''' [http://www.atcc.org/Products/All/208526.aspx ATCC:208526]
Line 81: Line 81:
 
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
  
'''References:''' [http://db.yeastgenome.org/reference/S000054242/overview Broach et al.] (1979) Gene 8:121-133
+
'''References:''' [http://www.yeastgenome.org/reference/S000054242/overview Broach et al.] (1979) Gene 8:121-133
  
 
==X2180-1A==
 
==X2180-1A==
Line 88: Line 88:
 
'''Notes:'''S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180
 
'''Notes:'''S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180
  
'''References:'''  [http://db.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston]  
+
'''References:'''  [http://www.yeastgenome.org/reference/S000050744/overview Mortimer and Johnston]  
  
 
'''Sources:''' [http://www.atcc.org/Products/All/204504.aspx ATCC:204504]
 
'''Sources:''' [http://www.atcc.org/Products/All/204504.aspx ATCC:204504]
  
==XJ24-24a==
+
=CEN.PK (aka CEN.PK2)=
'''Genotype:''' ''MAT''a ''ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2''
+
'''Genotype:''' ''MAT''a/α'' ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 ''Δ''1/his3 ''Δ''1 MAL2-8C/MAL2-8C SUC2/SUC2''
 +
 
 +
'''Notes:''' CEN.PK  possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance ([http://www.yeastgenome.org/reference/S000052724/overview Vanhalewyn et al., 1999]; [http://www.yeastgenome.org/reference/S000043601/overview Dumortier et al., 2000]).
 +
 
 +
'''References:''' [http://www.yeastgenome.org/reference/S000079648/overview van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714
 +
 
 +
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/cen.html EUROSCARF:30000D]
 +
 
 +
=D273-10B=
 +
'''Genotype:''' ''MAT''α ''mal''
 +
 
 +
'''Notes:''' Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.
 +
 
 +
'''References:''' [http://www.yeastgenome.org/reference/13977171/overview Sherman, F.] (1963) Genetics 48:375-385.
 +
 
 +
'''Sources:''' [http://www.atcc.org/Products/All/24657.aspx ATCC:24657]
 +
 
 +
=FL100=
 +
'''Genotype:''' ''MAT''a
 +
 
 +
'''References:''' [http://www.yeastgenome.org/reference/S000065623/overview Lacroute, F.] (1968) J. Bacteriol. 95:824-832.
 +
 
 +
Sources: [http://www.atcc.org/Products/All/28383.aspx ATCC:28383]
 +
 
 +
=JK9-3d=
 +
 
 +
There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:
 +
 
 +
'''Genotypes:''' JK9-3da  ''MAT''a ''leu2-3,112 ura3-52 rme1 trp1 his4''
 +
 
 +
JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locus
 +
 
 +
JK9-3da/α is homozygous for all markers except mating type
 +
 
 +
'''Notes:''' JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching.  It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.
 +
 
 +
Recent work shows that JK9-3d carries an ''rme1'' mutation that may be responsible for the rapid G1 arrest of this strain upon exposure to rapamycin ([http://www.yeastgenome.org/reference/S000181599/overview Moreno-Torres M, et al. (2015) Nat Commun 6:8256])
  
'''Notes:''' Derived from, but not isogenic to, S288C
+
'''References:''' [http://www.yeastgenome.org/reference/S000054286/overview Heitman et al.] (1991a) Science 253(5022):905-9 and [http://www.yeastgenome.org/reference/S000054822/overview Heitman et al.] (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
  
'''References:''' [http://db.yeastgenome.org/reference/S000055409/overview Strathern et al.] (1979) Cell 18:309-319
+
=RM11-1a=
  
=YNN216=
+
'''Genotype:''' ''MAT''a ''leu2''Δ''0 ura3-''Δ''0 HO::kanMX
'''Genotype:''' ''MAT''a/&#x3B1; ''ura3-52/ura3-52 lys2-801<sup>amber</sup>/lys2-801<sup>amber</sup> ade2-101<sup>ochre</sup>/ade2-101<sup>ochre</sup>''
 
  
'''Notes:''' Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).
+
'''Notes:''' RM11-1a is a haploid derivative of RM11, which is a diploid derivative of Bb32(3), which is an ascus derived from Bb32, which is a natural isolate collected by Robert Mortimer from a California vineyard (Ravenswood Zinfandel) in 1993, as in [http://www.yeastgenome.org/reference/S000041556/overview Mortimer et al.] (1994). It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the [http://www.broadinstitute.org/annotation/genome/saccharomyces_cerevisiae.3/Home.html Broad Institute website].
  
'''References:''' [http://db.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.
+
'''References:''' [http://www.yeastgenome.org/reference/S000069875/overview Brem et al.] (2002) Science 296(5568):752-5
==YPH499==
+
=SEY6210/SEY6211=
'''Genotype:''' ''MAT''a ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
+
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; ''leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-''&#x394;''200/his3-''&#x394;''200 trp1-''&#x394;''901/trp1-''&#x394;''901 ade2/ADE2 suc2-''&#x394;''9/suc2-''&#x394;''9 GAL/GAL LYS2/lys2-801''
  
'''Notes:''' Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that ''trp1-''&#x394;''63'', unlike ''trp1-''&#x394;''1'', does not delete adjacent ''GAL3'' UAS sequence and retains homology to ''TRP1'' selectable marker. ''gal2-'', does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
+
'''Notes:''' SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.
  
'''References:''' [http://db.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
+
'''References:''' [http://www.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
  
'''Sources:''' [http://www.atcc.org/Products/All/204679.aspx ATCC:204679]
+
'''Sources:''' [http://www.atcc.org/Products/All/201392.aspx ATCC:201392]
  
==YPH500==
+
==SEY6210==
'''Genotype:''' ''MAT''&#x3B1; ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
+
'''Genotype:''' ''MAT''&#x3B1; ''leu2-3,112 ura3-52 his3-''&#x394;''200 trp1-''&#x394;''901 suc2-''&#x394;''9 lys2-801; GAL''
  
'''Notes:'''''MAT''&#x3B1; strain isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
+
'''Notes:''' SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.
  
'''References:''' [http://db.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
+
'''References:''' [http://www.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
  
'''Sources:''' [http://www.atcc.org/Products/All/204680.aspx ATCC:204680]
+
'''Sources:''' [http://www.atcc.org/Products/All/96099.aspx ATCC:96099]
  
==YPH501==
+
==SEY6211==
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; ''ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-''&#x394;''63/trp1-''&#x394;''63 his3-''&#x394;''200/his3-''&#x394;''200 leu2-''&#x394;''1/leu2-''&#x394;''1''
+
'''Genotype:''' ''MAT''a ''leu2-3,112 ura3-52 his3-''&#x394;''200 trp1-''&#x394;''901 ade2-101 suc2-''&#x394;''9; GAL''
  
'''Notes:''' a/&#x3B1; diploid isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] and [http://staff.yeastgenome.org/index.php/straintable#yph500 YPH500]. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
+
'''Notes:''' SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.
  
'''References:''' [http://db.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
+
'''References:''' [http://www.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
  
'''Sources:''' [http://www.atcc.org/Products/All/204681.aspx ATCC:204681]
+
'''Sources:''' [http://www.atcc.org/Products/All/96100.aspx ATCC:96100]
  
 
=Sigma1278b=
 
=Sigma1278b=
Line 137: Line 172:
  
 
[http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000823 Granek and Magwene], PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of the Sigma1278B background contain
 
[http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000823 Granek and Magwene], PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of the Sigma1278B background contain
a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology. The Sigma1278b genome is closely related to S288c, and shares some other genomic regions with W303 [http://dx.doi.org/10.1098/rsob.120093].
+
a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology. The Sigma1278b genome is closely related to S288c, and shares some other genomic regions with W303 [http://rsob.royalsocietypublishing.org/content/2/8/120093].
 
 
Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found [http://mcdb.colorado.edu/labs1/dowelllab/pubs/DowellRyan/ here].
 
  
 +
Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).
 
=SK1=
 
=SK1=
 
'''Genotype:''' ''MAT''a/&#x3B1;'' HO gal2 cup<sup>S</sup> can1<sup>R</sup> BIO''
 
'''Genotype:''' ''MAT''a/&#x3B1;'' HO gal2 cup<sup>S</sup> can1<sup>R</sup> BIO''
Line 148: Line 182:
 
The SK1 genome was sequenced at the [http://www.sanger.ac.uk/research/projects/genomeinformatics/ Sanger Institute].
 
The SK1 genome was sequenced at the [http://www.sanger.ac.uk/research/projects/genomeinformatics/ Sanger Institute].
  
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079650 Kane SM and Roth J.] (1974) Bacteriol. 118: 8-14
+
'''References:''' [http://www.yeastgenome.org/reference/S000079650/overview Kane SM and Roth J.] (1974) Bacteriol. 118: 8-14
  
 
'''Sources:''' [http://www.atcc.org/Products/All/204722.aspx ATCC:204722]
 
'''Sources:''' [http://www.atcc.org/Products/All/204722.aspx ATCC:204722]
 
=CEN.PK (aka CEN.PK2)=
 
'''Genotype:''' ''MAT''a/&#x3B1;'' ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 ''&#x394;''1/his3 ''&#x394;''1 MAL2-8C/MAL2-8C SUC2/SUC2''
 
 
'''Notes:''' CEN.PK  possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance ([http://www.yeastgenome.org/reference/S000052724/overview Vanhalewyn et al., 1999]; [http://www.yeastgenome.org/reference/S000043601/overview Dumortier et al., 2000]).
 
 
'''References:''' [http://db.yeastgenome.org/reference/S000079648/overview van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714
 
 
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/cen.html EUROSCARF:30000D]
 
  
 
=W303=
 
=W303=
Line 215: Line 240:
 
   
 
   
  
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').<br />''bud4'' info: [http://www.yeastgenome.org/reference/S000120449/overview Voth et al.] (2005) Eukaryotic Cell, 4:1018-28.<br />''rad5-535'' info: see [[CommunityW303.html|detailed notes]]  
+
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').<br />''bud4'' info: Original mutant description [http://www.yeastgenome.org/reference/S000120449/overview Voth et al.] (2005) Eukaryotic Cell, 4:1018-28. Mutation: deletion of one of four Gs at positions 2456-2459 of BUD4 ORF. Seq data from: Ralser et al above<br />''rad5-535'' info: see [[CommunityW303.html|detailed notes]]  
  
'''Sources:''' [http://www.thermoscientificbio.com/search/?term=YSC1058 Thermo Scientific:YSC1058]
+
'''Sources:''' [http://www.atcc.org/Products/All/200060.aspx ATCC:200060]
  
 
==W303-1A==
 
==W303-1A==
Line 224: Line 249:
 
'''Notes:''' W303-1A possesses a ''ybp1-1'' mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.
 
'''Notes:''' W303-1A possesses a ''ybp1-1'' mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.
  
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').<br />''ybp1-1'' info: [http://db.yeastgenome.org/reference/S000073844/overview Veal et al.] (2003) J. Biol. Chem. 278:30896-904.<br />
+
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').<br />''ybp1-1'' info: [http://www.yeastgenome.org/reference/S000073844/overview Veal et al.] (2003) J. Biol. Chem. 278:30896-904.<br />
  
'''Sources:''' [http://www.thermoscientificbio.com/search/?term=YSC1058 Thermo Scientific:YSC1058]
+
'''Sources:''' [http://www.atcc.org/products/all/208352.aspx ATCC:208352]
  
 
==W303-1B==
 
==W303-1B==
Line 233: Line 258:
 
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').
 
'''References:''' W303 constructed by Rodney Rothstein (''see [[CommunityW303.html|detailed notes]] from RR and Stephan Bartsch'').
  
'''Sources:''' [http://www.thermoscientificbio.com/search/?term=YSC1058 Thermo Scientific:YSC1058]
+
'''Sources:''' [http://www.atcc.org/Products/All/201238.aspx ATCC:201238]
  
 
==W303-K6001==
 
==W303-K6001==
Line 247: Line 272:
 
'''References:''' [http://www.yeastgenome.org/reference/S000040392/overview Askwith C, et al.] (1994) Cell 76(2):403-10 PMID: 8293473
 
'''References:''' [http://www.yeastgenome.org/reference/S000040392/overview Askwith C, et al.] (1994) Cell 76(2):403-10 PMID: 8293473
  
=D273-10B=
+
=XJ24-24a=
'''Genotype:''' ''MAT''&#x3B1; ''mal''
+
'''Genotype:''' ''MAT''a ''ho HMa HM&#x3B1; ade6 arg4-17 trp1-1 tyr7-1 MAL2''
  
'''Notes:''' Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.
+
'''Notes:''' Likely quite different from S288C. A strain derived from XJ24-24a called XG1#24 had a recombination between HML and MAT that generated a large ring chromosome (Strathern et al. 1979 Cell), and Carol Newlon generated an ordered map of plasmid sub clones from this ring chromosome (Newlon et al. 1991 Genetics) that was then used for the initial sequencing of Chromosome III (Oliver et al. 1992), which has since been updated numerous times.  The provenance of XJ24-24a is unclear. Newlon was able to trace it back about 5 generations: some of the progenitor strains were from the Cold Spring Harbor Yeast course, and some of those strains had some markers similar to S288C (none of which are still in XJ24-24a). <br>''Thanks to Joachim Li for sharing this history of XJ24-24a with SGD.''
  
'''References:''' [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000080158 Sherman, F.] (1963) Genetics 48:375-385.
+
'''References:'''  
 +
* [http://www.yeastgenome.org/reference/S000055409/overview Strathern et al.] (1979) Cell 18:309-319
 +
* [http://www.yeastgenome.org/reference/S000055743/overview Newlon et al.] (1979) Genetics 129:343-57
 +
* [http://www.yeastgenome.org/reference/S000060078/overview Oliver et al.] (1992) Nature 357:38-46
  
'''Sources:''' [http://www.atcc.org/Products/All/24657.aspx ATCC:24657]
+
=Y55=
  
=FL100=
+
'''Genotype:''' ''MAT''a /''MAT''alpha  ''HO''/''HO''
'''Genotype:''' ''MAT''a
 
  
'''References:''' [http://db.yeastgenome.org/reference/S000065623/overview Lacroute, F.] (1968) J. Bacteriol. 95:824-832.
+
'''Notes:''' Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate non-auxotrophs. Y55 background strains have been used to study the timing of meiotic recombination ([http://www.yeastgenome.org/reference/S000148282/overview Borts et al. 1984]); to isolate almost all the subunits of the proteasome ([http://www.yeastgenome.org/reference/3294104/overview McCusker and Haber 1988a], [http://www.yeastgenome.org/reference/3294103/overview 1988b]); to get mutations in PMA1 and related genes ([http://www.yeastgenome.org/reference/2963211/overview McCusker 1986]); and to do meiotic mapping and interference experiments ([http://www.yeastgenome.org/reference/15454526/overview Malkova et al. 2004]).
 +
=YNN216=
 +
'''Genotype:''' ''MAT''a/&#x3B1; ''ura3-52/ura3-52 lys2-801<sup>amber</sup>/lys2-801<sup>amber</sup> ade2-101<sup>ochre</sup>/ade2-101<sup>ochre</sup>''
  
Sources: [http://www.atcc.org/Products/All/28383.aspx ATCC:28383]
+
'''Notes:''' Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).
  
=SEY6210/SEY6211=
+
'''References:''' [http://www.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://www.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; ''leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-''&#x394;''200/his3-''&#x394;''200 trp1-''&#x394;''901/trp1-''&#x394;''901 ade2/ADE2 suc2-''&#x394;''9/suc2-''&#x394;''9 GAL/GAL LYS2/lys2-801''
+
==YPH499==
 +
'''Genotype:''' ''MAT''a ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
  
'''Notes:''' SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.
+
'''Notes:''' Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that ''trp1-''&#x394;''63'', unlike ''trp1-''&#x394;''1'', does not delete adjacent ''GAL3'' UAS sequence and retains homology to ''TRP1'' selectable marker. ''gal2-'', does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
  
'''References:''' [http://db.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
+
'''References:''' [http://www.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://www.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://www.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
  
'''Sources:''' [http://www.atcc.org/Products/All/201392.aspx ATCC:201392]
+
'''Sources:''' [http://www.atcc.org/Products/All/204679.aspx ATCC:204679]
  
==SEY6210==
+
==YPH500==
'''Genotype:''' ''MAT''&#x3B1; ''leu2-3,112 ura3-52 his3-''&#x394;''200 trp1-''&#x394;''901 suc2-''&#x394;''9 lys2-801; GAL''
+
'''Genotype:''' ''MAT''&#x3B1; ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
 
 
'''Notes:''' SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.
 
  
'''References:''' [http://db.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
+
'''Notes:'''''MAT''&#x3B1; strain isogenic to [http://wiki.yeastgenome.org/index.php/Commonly_used_strains#YPH499 YPH499] except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
  
'''Sources:''' [http://www.atcc.org/Products/All/96099.aspx ATCC:96099]
+
'''References:''' [http://www.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://www.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://www.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
  
==SEY6211==
+
'''Sources:''' [http://www.atcc.org/Products/All/76626.aspx ATCC:76626]
'''Genotype:''' ''MAT''a ''leu2-3,112 ura3-52 his3-''&#x394;''200 trp1-''&#x394;''901 ade2-101 suc2-''&#x394;''9; GAL''
 
  
'''Notes:''' SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.
+
==YPH501==
 +
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; ''ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-''&#x394;''63/trp1-''&#x394;''63 his3-''&#x394;''200/his3-''&#x394;''200 leu2-''&#x394;''1/leu2-''&#x394;''1''
  
'''References:''' [http://db.yeastgenome.org/reference/S000045321/overview Robinson et al.] (1988) Mol Cell Biol 8(11):4936-48
+
'''Notes:''' a/&#x3B1; diploid isogenic to [http://wiki.yeastgenome.org/index.php/Commonly_used_strains#YPH499 YPH499] and [http://wiki.yeastgenome.org/index.php/Commonly_used_strains#YPH500 YPH500]. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
  
'''Sources:''' [http://www.atcc.org/Products/All/96100.aspx ATCC:96100]
+
'''References:''' [http://www.yeastgenome.org/reference/S000044428/overview Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://www.yeastgenome.org/reference/S000042217/overview Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://www.yeastgenome.org/reference/6092912/overview Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
  
=JK9-3d=
+
'''Sources:''' [http://www.atcc.org/Products/All/204681.aspx ATCC:204681]
 
 
There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:
 
 
 
'''Genotypes:''' JK9-3da  ''MAT''a ''leu2-3,112 ura3-52 rme1 trp1 his4''
 
 
 
JK9-3d&#x3B1; has the same genotype as JK9-3da with the exception of the MAT locus
 
 
 
JK9-3da/&#x3B1; is homozygous for all markers except mating type
 
 
 
'''Notes:''' JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and
 
derived the a/alpha isogenic diploid by mating type switching.  It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.
 
 
 
'''References:''' [http://db.yeastgenome.org/reference/S000054286/overview Heitman et al.] (1991a) Science 253(5022):905-9 and [http://db.yeastgenome.org/reference/S000054822/overview Heitman et al.] (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
 
 
 
=RM11-1a=
 
 
 
'''Genotype:''' ''MAT''a ''leu2''&#x394;''0 ura3-''&#x394;''0 HO::kanMX
 
 
 
'''Notes:''' RM11-1a is a haploid derivative of RM11, which is a diploid derivative of Bb32(3), which is an ascus derived from Bb32, which is a natural isolate collected by Robert Mortimer from a California vineyard (Ravenswood Zinfandel) in 1993, as in [http://www.yeastgenome.org/reference/S000041556/overview Mortimer et al.] (1994). It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the [http://www.broadinstitute.org/annotation/genome/saccharomyces_cerevisiae.3/Home.html Broad Institute website].
 
 
 
'''References:''' [http://www.yeastgenome.org/reference/S000069875/overview Brem et al.] (2002) Science 296(5568):752-5
 
 
 
=Y55=
 
 
 
'''Genotype:''' ''MAT''a /''MAT''alpha  ''HO''/''HO''
 
 
 
'''Notes:''' Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate non-auxotrophs. Y55 background strains have been used to study the timing of meiotic recombination ([http://www.yeastgenome.org/reference/S000148282/overview Borts et al. 1984]); to isolate almost all the subunits of the proteasome ([http://www.yeastgenome.org/reference/3294104/overview McCusker and Haber 1988a], [http://www.yeastgenome.org/reference/3294103/overview 1988b]); to get mutations in PMA1 and related genes ([http://www.yeastgenome.org/reference/2963211/overview McCusker 1986]); and to do meiotic mapping and interference experiments ([http://www.yeastgenome.org/reference/15454526/overview Malkova et al. 2004]).
 

Revision as of 15:32, 30 October 2015

This page describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:

  • Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
  • van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
  • Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains


S288C

Genotype: MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.

The S288C genome was recently resequenced at the Sanger Institute.

References: Mortimer and Johnston (1986) Genetics 113:35-43.

Sources: ATCC:204508

BY4743

Genotype: MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As in S288c, this strain as well as haploid derivatives BY4741, and BY4742 have allelic variants of MIP1, SAL1 and CAT5 and these polymorphisms, described in the respective locus history notes for these genes (MIP1, SAL1 and CAT5) all contribute to the high observed petite frequency. Details regarding the contributions of these variants to petite formation are referenced in Dimitrov et al. (2009) Genetics 183(1):365-83. See the Brachmann et al., 1998 reference for details of strain construction.

References: Brachmann et al. (1998) Yeast 14:115-32.

Sources: ATCC:201390

FY4

Genotype: MATa

Notes: Derived from S288C.

References: Winston et al. (1995) Yeast 11:53-55.

Brachmann et al. (1998) Yeast 14:115-32.

DBY12020

Genotype: MATa(PGAL10+gal1)Δ::loxP, leu2Δ0::PACT1-GEV-NatMX, gal4Δ::LEU2, HAP1+

Notes: Derived from FY4.

Reference: McIsaac et al. (2011) Mol Biol Cell 22(22):4447-59.

DBY12021

Genotype: MATα(PGAL10+gal1)Δ::loxP, leu2Δ0::PACT1-GEV-NatMX, gal4Δ::LEU2, HAP1+

Notes: Derived from FY4.

Reference: McIsaac et al. (2011) Mol Biol Cell 22(22):4447-59.

FY1679

Genotype: MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Winston et al. (1995) Yeast 11:53-55.

Sources: EUROSCARF:10000D

AB972

Genotype: MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.

References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

Sources: ATCC:204511

A364A

Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO

Notes: Used in the systematic sequencing project, the sequence stored in SGD.

References: Hartwell (1967) J. Bacteriol. 93:1662-1670.

Sources: ATCC:208526

DC5

Genotype: MATa leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Broach et al. (1979) Gene 8:121-133

X2180-1A

Genotype: MATa SUC2 mal mel gal2 CUP1

Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180

References: Mortimer and Johnston

Sources: ATCC:204504

CEN.PK (aka CEN.PK2)

Genotype: MATa/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8C SUC2/SUC2

Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance (Vanhalewyn et al., 1999; Dumortier et al., 2000).

References: van Dijken et al. (2000) Enzyme Microb Technol 26:706-714

Sources: EUROSCARF:30000D

D273-10B

Genotype: MATα mal

Notes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.

References: Sherman, F. (1963) Genetics 48:375-385.

Sources: ATCC:24657

FL100

Genotype: MATa

References: Lacroute, F. (1968) J. Bacteriol. 95:824-832.

Sources: ATCC:28383

JK9-3d

There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:

Genotypes: JK9-3da MATa leu2-3,112 ura3-52 rme1 trp1 his4

JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locus

JK9-3da/α is homozygous for all markers except mating type

Notes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.

Recent work shows that JK9-3d carries an rme1 mutation that may be responsible for the rapid G1 arrest of this strain upon exposure to rapamycin (Moreno-Torres M, et al. (2015) Nat Commun 6:8256)

References: Heitman et al. (1991a) Science 253(5022):905-9 and Heitman et al. (1991b) Proc Natl Acad Sci U S A 88(5):1948-52

RM11-1a

Genotype: MATa leu2Δ0 ura3-Δ0 HO::kanMX

Notes: RM11-1a is a haploid derivative of RM11, which is a diploid derivative of Bb32(3), which is an ascus derived from Bb32, which is a natural isolate collected by Robert Mortimer from a California vineyard (Ravenswood Zinfandel) in 1993, as in Mortimer et al. (1994). It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the Broad Institute website.

References: Brem et al. (2002) Science 296(5568):752-5

SEY6210/SEY6211

Genotype: MATa/MATα leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-Δ901/trp1-Δ901 ade2/ADE2 suc2-Δ9/suc2-Δ9 GAL/GAL LYS2/lys2-801

Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.

References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48

Sources: ATCC:201392

SEY6210

Genotype: MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801; GAL

Notes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.

References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48

Sources: ATCC:96099

SEY6211

Genotype: MATa leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2-101 suc2-Δ9; GAL

Notes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.

References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48

Sources: ATCC:96100

Sigma1278b

Notes: Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.

Granek and Magwene, PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of the Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology. The Sigma1278b genome is closely related to S288c, and shares some other genomic regions with W303 [1].

Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in Dowell et al. (2010).

SK1

Genotype: MATa/α HO gal2 cupS can1R BIO

Notes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative.

The SK1 genome was sequenced at the Sanger Institute.

References: Kane SM and Roth J. (1974) Bacteriol. 118: 8-14

Sources: ATCC:204722

W303

Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

allele
locus
mutation (1)
ade2-1
YOR128C nonsense, glu64STOP
trp1-1
YDR007W nonsense, glu83STOP
can1-100
YEL063C frameshift, lys47
leu2-3,112
YCL018W
frameshift, gly83
his3-11,15
YOR202W 2x frameshifts, ala70 and glu106

Notes: The W303 genome is to 85.4% derived from S288c, part of the other regions are similar to non-S288c regions of Sigma1278b. In total, some 800 CDS differ between W303 and S288c, but in most cases only one or two residues differ [2]. These include a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variant of MIP1 which increases petite frequency.

The W303 genome was sequenced at the Sanger Institute and by Ralser M. et al. (2012) Open Biol 2: 120093. 1 (DDBJ/EMBL/GenBank ALAV00000000).


References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).
bud4 info: Original mutant description Voth et al. (2005) Eukaryotic Cell, 4:1018-28. Mutation: deletion of one of four Gs at positions 2456-2459 of BUD4 ORF. Seq data from: Ralser et al above
rad5-535 info: see detailed notes

Sources: ATCC:200060

W303-1A

Genotype: MATa {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).
ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.

Sources: ATCC:208352

W303-1B

Genotype: MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).

Sources: ATCC:201238

W303-K6001

Genotype: MATa; {ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG, ura3::URA3 GAL-ubiR-CDC6 (at URA3)}

References: K6001 was created in Kim Nasmyth's lab Piatti at al (PMID: 7641697) and Bobola et al (PMID: 8625408). K6001 has become a popular model in yeast aging research, as it allows a replicative aging assay based on microcolonies (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566), Ralser et al [3].

DY1457

Genotype: MATa; {ade6 can1-100(oc) his3-11,15 leu2-3,112 trp1-1 ura3-52}

References: Askwith C, et al. (1994) Cell 76(2):403-10 PMID: 8293473

XJ24-24a

Genotype: MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Likely quite different from S288C. A strain derived from XJ24-24a called XG1#24 had a recombination between HML and MAT that generated a large ring chromosome (Strathern et al. 1979 Cell), and Carol Newlon generated an ordered map of plasmid sub clones from this ring chromosome (Newlon et al. 1991 Genetics) that was then used for the initial sequencing of Chromosome III (Oliver et al. 1992), which has since been updated numerous times. The provenance of XJ24-24a is unclear. Newlon was able to trace it back about 5 generations: some of the progenitor strains were from the Cold Spring Harbor Yeast course, and some of those strains had some markers similar to S288C (none of which are still in XJ24-24a).
Thanks to Joachim Li for sharing this history of XJ24-24a with SGD.

References:

Y55

Genotype: MATa /MATalpha HO/HO

Notes: Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate non-auxotrophs. Y55 background strains have been used to study the timing of meiotic recombination (Borts et al. 1984); to isolate almost all the subunits of the proteasome (McCusker and Haber 1988a, 1988b); to get mutations in PMA1 and related genes (McCusker 1986); and to do meiotic mapping and interference experiments (Malkova et al. 2004).

YNN216

Genotype: MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.

YPH499

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204679

YPH500

Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:76626

YPH501

Genotype: MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1

Notes: a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204681