YDR043C

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Systematic name YDR043C
Gene name NRG1
Aliases
Feature type ORF, Verified
Coordinates Chr IV:543369..542674
Primary SGDID S000002450


Description of YDR043C: Transcriptional repressor that recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response[1][2][3][4]




Contents

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [5] [6]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [5] [6]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [5] [6]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [5] [6]


Overexpression

Phenotype(s): Dominant, Gain of function

Overexpression of VP16-Nrg1 results in partial derepression of SUC2 in glucose grown cells. [7] [8]


Interactions

Genetic

YDR043C is a suppressor of Snf1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg1 partially suppresses Snf1's defect in invasive growth, FLO11 expression, and STA2 expression. [1] [8]


Other Interactions

Other Topic: functional dependence Other Interactions interaction with Tup1
Repression by LexA-Nrg1 of a heterologous promoter containing lexA binding sites depends on Tup1. [3] [9]


Other Topic: functional dependence Other Interactions interaction with Cyc8
Repression by LexA-Nrg1 of a heterologous promoter containing lexA binding sites depends on Cyc8/Ssn6. [3] [9]


Physical

Physical interaction with Cyc8
Nrg1 interacts with Ssn6/Cyc8 in the two-hybrid system, and in GST pull-down experiments. [3] [9]


Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg1 [7] [8]


Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg1 [7] [8]


Regulatory

Regulatory interaction with STA1
Nrg1 binds to the promoter of STA1, where it represses transcription of this gene. [3] [9]


Two Hybrid

Two Hybrid interaction with snf1
Snf1 kinase domain interacts with Nrg1 [7] [8]


Protein Details

Nucleic Acid Binding

Nrg1 specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. [3] [9]


Protein Function/Process

LexA-Nrg1 represses transcription from a heterologous promoter containing LexA binding sites. [7] [8]


Protein Localization

Nrg1 is nuclear in both YP 2% Glucose and YP 2%Glycerol/2% Ethanol [7] [8]





References

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  1. 1.0 1.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
  2. Lamb TM and Mitchell AP (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23(2):677-86 SGD PMID 12509465
  3. 3.0 3.1 3.2 3.3 3.4 3.5 Park SH, et al. (1999) Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. Mol Cell Biol 19(3):2044-50 SGD PMID 10022891
  4. Zhou H and Winston F (2001) NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae. BMC Genet 2():5 SGD PMID 11281938
  5. 5.0 5.1 5.2 5.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  6. 6.0 6.1 6.2 6.3 submitted by Valmik K. Vyas on 2003-03-27
  7. 7.0 7.1 7.2 7.3 7.4 7.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 SGD PMID 11404322
  8. 8.0 8.1 8.2 8.3 8.4 8.5 8.6 submitted by Valmik K. Vyas on 2003-02-04
  9. 9.0 9.1 9.2 9.3 9.4 submitted by Valmik K. Vyas on 2003-02-12

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